Coding

Part:BBa_K2593003:Design

Designed by: Huimin Li   Group: iGEM18_SSTi-SZGD   (2018-09-20)



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In Bacillus subtilis, there are two TatAC-type systems, TatAdCd and TatAyCy, operate in parallel with different substrate specificities. in our project AmyX was fused in N-terminal of the LHyal gene for the extracellular export of the expressed protein


Source

AmyX is an a-Amylase protein encoding gene and is originated from B.amyloliquefaciens.